M.J. and the Tigers
The specter of Mary Jane Watson hangs over this film much more than either the spider-bite or Uncle Ben. In the comic books, Peter didn't get tied up in a M.J./Gwen Stacy love triangle until college (and that was after both Betty Brant and Liz Allen dated him and/or expressed total disinterest in ever dating him). The Sam Raimi movies brought M.J. into Peter's life from beginning, and the Marc Webb movies killed off Gwen before she could even get to college (plus Mary Jane was played by Shailene Woodley, but all of her scenes wound up on the cutting room floor).
Homecoming doesn't reveal it's M.J. until the very end of the film, but is hinting at her presence the whole way through. The mascot of Midtown high happens to be a tiger, and we finally see a kid dressed as the costume character as the shot leading into the movie's big M.J. reveal, both a play on Mary Jane of the comics calling Peter "tiger." Here, it's revealed that Michelle, played by Zendaya, likes her friends to call her "M.J." This likely means that for the first time in Spider-Man history, M.J. won't be "Mary Jane" but Michelle….whatever her last name ends up being. What if it's Jamison? That'd be a hoot.